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94
MedChemExpress dbco peg 3 vc pab mmae 5
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Dbco Peg 3 Vc Pab Mmae 5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation lenalidomide 4'-peg3-amine
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Lenalidomide 4' Peg3 Amine, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Croda International Plc ammonium salt pa peg3 mannose 16 0
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Ammonium Salt Pa Peg3 Mannose 16 0, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress dbco peg3 vc pab mmae 5
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Dbco Peg3 Vc Pab Mmae 5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress fbng
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Fbng, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher j64996 streptavidin beads pierce
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
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Thermo Fisher azide polyethylene glycol biotin conjugate thermofisher
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Azide Polyethylene Glycol Biotin Conjugate Thermofisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Croda International Plc dipalmitoyl sn glycero 3 phospho
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
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MedChemExpress tamra peg3 n3
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
Tamra Peg3 N3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Croda International Plc 1 2 dipalmitoyl sn glycero 3 phospho l serine
Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8
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Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8

Journal: ACS Omega

Article Title: Site-Specific Glycan Conjugation Improves Stability and Efficacy of an Antibody–Drug Conjugates Bearing DXd as a Cytotoxic Payload

doi: 10.1021/acsomega.5c10898

Figure Lengend Snippet: Preparation Scheme of Glycan-Conjugated ADC and Payload Structures. (i) Inherent Heterogeneous Glycan Removal by ENGase. ENGase Cleaves the Bond Between the β-1,4-Glycosidic Linkage Within the N,N ′-Diacetylchitobiose Core. Heterogeneous Regions are Indicated in Parentheses; (ii) An Azide-Carrying Glycan 4 was Added via the ENGase Mutant; (iii) Biorthogonal Reaction Between the Azide Group and DBCO. Payload Structure: DBCO-PEG 3 -VC-PAB-MMAE 5 ; DBCO-PEG 4 -GGFG-DXd 6 ; Trasuzumab-SG-PEG 3 -VC-PAB-MMAE 7 ; Trasuzumab-SG-PEG 4 -GGFG-DXd 8

Article Snippet: DBCO-PEG 3 -VC-PAB-MMAE 5 and DBCO-PEG 4 -Gly-Gly-Phe-Gly-DXd 6 were purchased from MedChemExpress (Monmouth Junction, NJ, USA) and Levena Biopharma (San Diego, CA, USA), respectively.

Techniques: Glycoproteomics, Mutagenesis